Progressive tumor features accompany epithelial-mesenchymal transition induced in mitochondrial DNA-depleted cells.

CANCER SCIENCE(2008)

Cited 69|Views19
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Abstract
The growth of LNCaP, a human prostate adenocarcinoma cell line, and MCF-7, a human breast adenocarcinoma cell line, is initially hormone dependent. We previously demonstrated that LN rho 0-8 and MCF rho 0, derived from LNCaP and MCF-7 by depleting mitochondrial DNA (mtDNA), exhibited hormone-independent growth that led to progressed phenotypes. Here, we demonstrate that LN rho 0-8 and MCF rho 0 have invasive characters as evaluated by the ability of invasion through the extracellular matrix (ECM) in vitro. In addition, the induction of vimentin and the repression of E-cadherin expression in rho 0 cells indicate that they are mesenchymal cells. Since LN rho 0-8 and MCF rho 0 were derived from epithelial cancer cell lines, LNCaP and MCF-7 must have lost epithelial features and gained the mesenchymal phenotype by epithelial-mesenchymal transition (EMT) during the mtDNA depletion. In the rho 0 cell lines, the Raf/MAPK signaling cascade was highly activated together with the expressions of transforming growth factor-beta (TGF-beta) and type I TGF-beta receptor (TGF-beta RI). EMT requires cooperation of TGF-beta signaling with activation of the Raf/MAPK cascade, suggesting that EMT was induced in mtDNA depleted cells resulting in the acquisition of progressive tumor features, such as higher invasiveness and loss of hormone dependent growth. Our results indicate that decreasing mtDNA content induces EMT, enabling the progressive phenotypes observed in cancer. (Cancer Sci 2008; 99: 1584-1588)
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Key words
cadherins,signal transduction,epithelial mesenchymal transition,mitochondrial dna,transforming growth factor beta,vimentin,phenotype
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