Lipid-soluble components from fig residue and its anti-tumor activities in vitro.

Extraction and purification technology of lipid-soluble anti-tumor active components from fig residues were studied. Thirteen crystals were got after column chromatography, spectrometric study and structural analysis. The anti-tumor activity of these 13 crystals was investigated on blood tumor cell line(U937), lung tumor cell line(95D)and stomach tumor cell line(AGS)in vitro. The Number 6 crystal was identified as β-sitosterol and validated as anti-tumor component for its fifty percent inhibition content (IC50)lower than 10mg/L. The optimum lipid-soluble anti-tumor components extraction condition are as follows, using petroleum ether(boiling point from 60 to 90℃) as solvent and the fig residues granularity 750μm, extracting temperature 85℃, extracting time 130min, the ratio of solid to solvent 1∶11(g∶mL), extracting twice. As a result the anti-tumor substance extraction yield is 23.1g/kg. The anti-tumor component was identified as β-sitosterol after column chromatography, spectrometric study and structural analysis.