Dissemination of catabolic plasmids among desiccation-tolerant bacteria in soil microcosms
Applied Biochemistry and Biotechnology(2001)
摘要
The dissemination of catabolic plasmids was compared to bioaugmentation by strain inoculation in microcosm experiments. When Rhodococcus erythropolis strain T902, bearing a plasmid with trich loroethene and isopropylbenzene degradation pathways, was used as the inoculum, no transconjugant was isolated but the strain remained in the soil. This plasmid had a narrow host range. Pseudomonas putida strain C8S3 was used as the inoculum in a second approach. It bore a broad host range conjugative plasmid harboring a natural transposon, RP4∶Tn4371, responsible for biphenyl and 4-chlorobiphenyl degradation pathways. The inoculating population slowly decreased from its original level (10 6 colony-forming units [CFU]/g of dry soil) to approx 3×10 2 CFU/g of dry soil after 3 wk. Transconjugant populations degrading biphenyl appeared in constant humidity soil (up to 2×10 3 CFU/g) and desiccating soil (up to 10 4 CFU/g). The feasibility of plasmid dissemination as a bioaugmentation technique was demonstrated in desiccating soils. The ecologic significance of desiccation in bioaugmentation was demonstrated; it upset the microbial ecology and the development of transconjugants.
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关键词
Bioaugmentation,drought tolerance,conjugation,plasmid dissemination,microcosm,isopropylbenzene
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