Rapid and quantitative detection of Brucella by up-converting phosphor technology-based lateral-flow assay

A rapid and quantitative up-converting phosphor technology-based later-flow assay (UPT-LF assay) was developed for on-site detection of Brucella. Different Brucella species both in pure cultures and in spiked samples could be quantitatively detected. The detection limit for pure culture was 5×106CFU/ml and the sensitivity for different spiked samples ranged from 2.0×103 to 3.9×105CFU/mg. The UPT-LF assay showed high specificity, reproducibility and stability, providing great potential for Brucella on-site detection.