Effects of 5-Aza-CdR on DLC-1 gene expression and biological behaviors of human colon cancer HT29 cell line

Objective To observe the effects of 5-Aza-2′-deoxycytidine(5-Aza-CdR) on the proliferation,invasion,migration ability and frequently deleted in liver cancer-1(DLC-1) gene expression of HT29 cell line.Methods The human colon cancer HT29 cell line was treated by various concertrations(5,10μmol/L) of 5-Aza-CdR for 72h.DLC-1 mRNA expression profile was studied by RT-PCR in pre-and post-treated cells.MTT assay was used for the test of cell proliferation.The invasion and mobility abilities of the cells in vitro were explored by colony-forming experiment and transwell chamber.Results Compared to the cell lines without treatment,the proliferation of HT29 cell lines cultivated with 5-Aza-CdR was decreased and the invasion and mobility abilities of the cells in vitro were lowered.The expression level of DLC-1 mRNA in HT29 was restored after treated by 5-Aza-CdR.Conclusion 5-Aza-CdR inhibits the malignant biological behaviors of HT29 cells,which may be related to the restoration of DLC-1 gene expression.