酵母还原型谷胱甘肽提取条件的优化
Food and Drug(2011)
Abstract
目的采用热水、乙醇、盐酸提取面包酵母中的还原型谷胱甘肽,获得最优的提取方法。方法在单因素试验的基础上对乙醇提取法的乙醇浓度、pH、温度、提取时间进行L9(34)正交试验并采用二次提取验证提取效果。结果在40℃,pH 1.0,乙醇含量50%条件下提取40 min,能有效地提取还原型谷胱甘肽,采用二次提取法验证其提取率达到95.4%。结论与其它方法相比,乙醇提取法是一种简便、快速、经济、有效的方法。
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Key words
ethanol,secondary extraction,glutathione,baker's yeast
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