ST段抬高型急性心肌梗死患者外周血心肌特异性微小RNA-208a水平的变化
Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue(2012)
Abstract
目的 观察心肌特异性微小RNA-208a(microRNA-208a,miR-208a)在ST段抬高型急性心肌梗死(STEAMI)患者血中的含量变化,探讨血miR-208a在STEAMI诊断中的作用.方法 采集30例STEAMI患者发病12h内的外周血,用实时荧光定量逆转录-聚合酶链反应(qRT-PCR)检测血中miR-208a相对含量,同时用酶联免疫吸附试验(ELISA)检测血清心肌肌钙蛋白Ⅰ(cTnⅠ)、肌酸激酶同工酶(CK-MB)含量.以30例健康者血标本作为对照.结果 STEAMI患者血cTnⅠ (μg/L)、CK-MB (μg/L)含量明显高于健康对照者(cTnⅠ:17.72±8.43比0.05±0.01,CK-MB:250.83±177.26比71.20±20.50,均P<0.01).在PCR 60个循环内,健康对照者血均未能检出miR-208a,STEAMI患者血中可检出miR-208a(0 ~6 h为44.95±4.77,6~ 12 h为43.98±4.68);以健康对照者血miR-208a阈值循环(Ct)值60为基础进行统计,AMI患者血miR-208a相对含量至少高于健康对照者215倍以上,两组比较差异有统计学意义(P<0.01).结论 血miR-208a可作为STEAMI患者早期诊断的新型敏感生物学标志.
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Key words
cardiac troponin i,mb isoenzyme of creatine kinase,microrna-208a,st segment elevation acute myocardial infarction
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