正交设计法优选翻白草中总黄酮的提取工艺
Journal of Medical Science Yanbian University(2008)
Abstract
[目的]探讨翻白草中总黄酮的最佳提取工艺.[方法]采用正交设计法以提取物中总黄酮含量为评价指标,以乙醇浓度、提取时间、固液比及提取次数为考察因素,筛选翻白草的最佳提取工艺.应用分光光度仪测定提取物中总黄酮的含量.[结果] 最佳提取条件为在乙醇浓度为700mL/L,固液比为1∶12,提取时间为150min,提取2次,其中乙醇用量为主要影响因素.[结论]所优选的提取工艺合理,操作简便,可行.
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Key words
extraction technology,flavones,plants,medicinal
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