油菜长链酰基辅酶A合成酶基因(LACS4)的电子克隆和表达分析
Shanghai（2008）
Abstract
长链酰基辅酶A合成酶(LACSs)把游离脂肪酸活化成为酰基辅酶A硫脂,在油脂的合成和降解途径中发挥着重要的作用。通过电子克隆的方法,发现了1个油菜(Brassica napus)LACS基因。该基因长2270 bp,包含2004 bp的开放阅读框,预测编码1个含有667个氨基酸残基的蛋白质,命名该蛋白为BnLACS4,序列分析显示,BnLACS4具有典型的LACS分子特征。表达分析显示BnLACS4在成熟油菜的根、茎、叶和花中都有表达。在授粉后(DAP)35 d,不同含油量的角果皮和种子中,BnLACS4的表达也存在差异。表达谱说明,BnLACS4可能参与油脂的生物合成以及油菜种子中的油脂积累。
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Key words
biochemistry,biological techniques,botany,enzymes,molecular biophysics,molecular configurations,sequences,BnLACS4 gene expression analysis,Brassica napus LACS gene analysis,acyl-CoA thioesters,amino acid residues,flowers,free fatty acid,in silicon cloning,leaves,lipid biosynthesis,lipid degradation,long chain acyl-coenzyme A synthetases,mature roots,molecular characteristics,oil accumulation,oil content varieties,open reading frame,pod coats,pollination,rapeseed,sequence analysis,stems
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