幸福树的组织培养与快速繁殖
Journal of Shandong Forestry Science and Technology(2009)
Abstract
以幸福树(Radermachera sinica)茎段为外植体,探讨不同植物激素6-BAI、BAI、AA的浓度对幸福树组培快繁的影响,目的是建立一套幸福树离体快繁培养的技术体系。试验结果表明:幸福树的初代培养为培养基MS+6-BA 0.8mg/L+IAA 0.1 mg/L+蔗糖30g/L+琼脂7.5 g/L,分化培养培养MS+6-BA 0.6g/L+IAA 0.2mg/L+蔗糖30g/L+琼脂7.5g/L,生根培养为1/2MS+IAA 0.1mg/L+糖30g/L+琼脂7.5g/L,通过正交试验也得出同样的结论。炼苗移栽时,土与蛭石(1:1)基质配比成活率最高,且生长旺盛。
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Key words
orthogonal tests plantlet,rapid propagation,Radermachera sinica,tissue culture
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