Biotechnological process for producing black bean slurry without stachyose

Beans are important sources of proteins and other nutrients. However, stachyose and other oligosaccharides (RFOs) are present in this legume causing flatulence (H2, CO2 and CH4), abdominal pain, and diarrhea. The problematic digestibility of these sugars in the small intestine is attributed to a lack of α-galactosidase, which is essential for the hydrolysis of α-1,6 linkages. The aim of the present work was to reduce the stachyose of black bean slurry by lactic acid fermentation using a selected Lactobacillus LPB56, an α-galactosidase producer. The bean slurry (6L) was fermented in a bioreactor with 1.3% (w/v) of CaCO3, at 37°C and 160rpm. Bacterial cells increased from 2.4×107 to 7.0×108CFU/mL, and the stachyose and other sugars were totally consumed after 18h of fermentation. The maximum activity of α-galactosidase was 0.162U/mL after 6h. The fermentative process did not cause significant changes on the composition of the bean product.