Effect of cigarette smoking on expression of inflammatory cytokines in acute myocardial infarction after successful thrombolysis

Objective:To clarify the effect of cigarette smoking on the expression levels of serum IL-1, hs-CRP, TNF-α and IL-10 in patients with acute myocardial infarction (AMI) before and after successful thrombolysis, and to explore the mechanism of the effect of cigarette smoking on the myocardial ischemia reperfusion injury (MIRI). Method:According to the smoking status, the AMI patients were divided into two groups, non smoking group and smoking group. All the patients were treated with recombinant streptokinase. The serum samples were collected before and after thrombolytic therapy at 12th hours and 24th hours respectively. Double antibody sandwich ABC ELISA was used to detect the expression levels of IL-1 and IL-10, the serum TNF-α and hs-CRP were measured by radioimmunoassay and rate nephelometry. Result:The serum levels of IL-1, hs-CRP, TNF-α and IL-10 of smoking group before and after intravenous thrombolysis reperfusion at 12th hours and 24th hours were all higher than those in non-smoking group (all P0.01). The four inflammatory cytokines levels after successful thrombosis at 12th hours and 24th hours were significantly higher than those before thrombolysis therapy (all P0.01), both in smoking group and non-smoking group. The four inflammatory cytokines levels after successful thrombosis at 12th hours and 24th hours had no statistical difference in non smoking group. Conclusion:Smoking can increase the levels of serum IL-1, hs-CRP, TNF-α and IL-10 in patients with AMI berore and after intravenous thrombolysis reperfusion, which caused the MIRI heavily, and it continued to increase the injury.