Effect of topiramate on neurological grade and evaluation neron-specific enolase after cerebral ischemic reperfusion of rats

[ Objective ] To study the expression and significance of neuron-specific enolase ( NSE) in the cerebral tissue after cerebral ischemic reperfusion damage and elucidate the protective mechanism of topiramate in cerebral ischemic brain damage. [ Method] 40 Wistar rats were randomly assigned to sham operated group, ischemia-reperfusion group, TPM treatd group with large-dose and TPM treated group with small-dose. All the rats were killed to collect the brain in 72 hours after the neural function value analysis were done. neurological grade evaluation was applied to examine the behavioral recovery and immunohistochemieal technique was used to detect the dynamic changes of NSE in cerebral tissue. [ Result ] There was an improvement in the score of neurologic grade in topiramate treatment group in the 72th hour after reperfusion compared with the contrails (P0. 01). The expression of NSE in topiramate treatment group increased than the fame-operation group in 72th hour after reperfusion (P 0. 01). In treatment group, the score of the neurologic grade of the large-dose group increased (P0. 05) , the expression of NSE protein increased remarkablely in cerebral tissue (P0. 05) compared with the small-dose group. [Conclusion]Topiramate could protect cerebral tissue against focal cerebral ischemic reperfusion damage whose role might be reflected by increasing the expression of NSE, and this neuroprotective effects is related to different dose of the medicine.