N-Linked Oligosaccharide Profiling By Hplc On Porous Graphitized Carbon (Pgc)

The vast array of possible N-linked oligosaccharides demands high-resolution HPLC columns for their purification (1,2). Reverse-phase (C18) and normal-phase (NH2) columns have been used for the separation (singly or in concert) of many N-linked oligosaccharides. The porous graphitized carbon (PGC) column described in Chapter 106 for O-linked alditol separation will give improved N-linked oligosaccharide resolution over C18 columns, and has the advantage of using salt-free buffers for preparative work (3,4).