笃斯越橘再生体系的建立

Journal of Northeast Agricultural University(2012)

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Abstract
以笃斯越橘休眠期的茎段和单芽为外植体,采用改良WPM为基本培养基,研究不同激素、外植体类型对植株再生和生根情况的影响。结果表明,枝条经过室内水培及10℃预处理,以单芽为外植体比以茎段为外植体可更有效快速地获得无菌苗。茎段及单芽的离体增殖生长的最适宜培养基为:WPM+1.5 mg.L-1ZT+0.3 mg.L-1NAA;生根最适宜培养基为:1/2WPM+1.5 mg.L-1IAA+0.1 mg.L-1IBA,生根成活率达82.3%。
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Key words
plant regeneration,Vaccinium uliginosum,tissue culture
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