Chrysanthemum Transformed with hpa1_(Xoo) Enhanced Resistance to Aphid

The gene hpa1Xoo encoding harpinXoo protein derived from Xathomonas oryzae pv.oryzae was inserted into transgenic vector pCAMBIA3300-bar.The leaf discs from chrysanthemum variety 'Han jv' QX-077 were infected by Agrobacterium tumefaciens strain LBA4404/pCAMBIA3300-hpa1Xoobar.Basta-resistant green shoots were obtained.PCR,Southern blot and RT-PCR analyses confirmed successful integration of the foreign gene into the genome of the 'Han jv' QX-077.Resistance bioassays of the leaves and plants of transgenic lines revealed that the transgenic plants could significantly inhibit the proliferation of Myzus persicae(t0.01).RT-PCR analysis confirmed that hpa1Xoo had expressed in transgenic 'han jv' plants at transcription level.Transformed chrysanthemum with hpa1Xoo gene could enhance the capability of aphid resistance.