不同培养方法对昆虫病原线虫质量影响及其评价
Plant Protection（2007）
Abstract
采用传统的White trap法、Lowtek培养法、液体和固体培养4种方法繁殖异小杆线虫[Heterorhabditis bac-teriophora(Hb-1)]和斯氏线虫[Steinernemacarpocapsae(Sc-2)],测定和计算了线虫的产量、质量和成本。试验结果显示,4种方法培养对线虫侵染力和存活率有一定影响,但差异不显著(p<0.05),2种线虫从高到低的顺序为:White trap法>Lowtek法>固体培养>液体培养;产量最高的是固体培养法,其次是液体培养法,Lowtek法最低;繁殖成本从低到高的顺序为:Lowtek法<固体培养More
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Key words
in vitro,mass production,entomopathogenic nematodes,evaluation,in vivo
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