The combined effects of high temperature and carbon monoxide on heat stress response
Journal of Tongji Medical University = Tong ji yi ke da xue xue bao(1995)
Abstract
Summary In this study, we have examined the effects of exposure to high temperature, carbon monoxide or a combination of both conditions
in a model system, the rat and in industrial workers. In the rat liver, HSP70 mRNA and HSP70 synthesis were measured by dot
hybridization and western blot. The results showed that after a heat stress HSP70 mRNA and its product, HSP70 increased significantly
and there was a synergism in the combined effects of high temperature and carbon monoxide exposure on the induction of HSP70
mRNA and HSP70 synthesis. Heat played a major role in this induction. The presence of antibodies to human HSP27, HSP60, HSP70,
HSC73, HSP89 α and β in workers exposed to heat, carbon monoxide was also measured by western blot using purified HSPs as
antigens. Plasma free amino acids were measured in the same group of workers. The incidence of antibodies to HSP27 and HSP70
was significantly higher in the workers working in an environment with extreme heat, and high carbon monoxide emission than
in a control group. The carbon monoxide exposed group showed the highest incidence of antibodies to HSPs. Although our previous
results indicated that workers had an insufficient protein intake, plasma free amino acids tended to increase, especially
in methionine and tryptophan two kinds of amino acids which are absent from the main stress protein, HSP70. These results
suggest that the major problems that these workers may face are how to facilitate the use of plasma free amino acids and reduce
the inhibition of synthesis of normal proteins when they are exposed to occupational harmful factors. These results also add
new information on the measurement of HSPs as a potential biomonitor to assess whether organisms are experiencing metabolic
stress within their environment.
MoreTranslated text
Key words
carbon monoxide,hsp70 mrna,plasma free amino acids,the combined effects,antibodies to heat stress proteins,hsp70,high temperature
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