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剔毒护肝方药物血清对肝星状细胞凋亡相关蛋白Bcl-2、Bax的影响

梁红梅,朱清静,杨玲,蔡燕萍,阮连国,周星,万十千,李霞,李传龙

CHINESE JOURNAL OF INTEGRATED TRADITIONAL AND WESTERN MEDICINE ON LIVER DISEASES（2009）

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Abstract
目的:研究剔毒护肝方药物血清对肝星状细胞凋亡相关蛋白Bcl-2、Bax的影响.方法:传代培养大鼠肝星状细胞(HSC)系HSC-T6与不同剂量剔毒护肝方(大、中、小剂量组)共同培养48小时,应用Annexin-V-碘化丙锭染色检测剔毒护肝方对大鼠HSC凋亡的影响;免疫组化检测凋亡相关蛋白Bcl-2、Bax水平.结果:剔毒护肝方能使大鼠HSC凋亡率明显增多,同时可使凋亡抑制分子Bcl-2表达下调,促凋亡分子Bax表达增强(P＜0.01).结论:剔毒护肝方可下调Bcl-2/Bax比率,促进HSC凋亡.
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hepatic stellate cells,tidu hugan fang,bax
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