柱前衍生化法测定莫西沙星小环的对映异构体
Food and Drug(2013)
Abstract
目的建立柱前衍生化法测定莫西沙星小环对映异构体的方法。方法采用Symmetry C18(4.6 mm×250 mm,5μm)色谱柱,流动相为甲醇-水(60:40),柱温为35℃;衍生试剂为2,3,4,6-四乙酰基-β-D-吡喃葡萄糖基异硫氰酸酯,检测波长为252 nm。结果莫西沙星小环对映体衍生物获得了基线分离,并确认了R,R-衍生物的色谱峰;浓度1.44~72 mg/L范围内与峰面积呈线性关系,回归方程为A=34 801C-6472(r=0.9999)。结论所建方法简便、快速,可用于莫西沙星小环对映异构体的拆分。
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Key words
2,glycopyranosyl isothiocyanate,enantioseparation,RP-HPLC,pre-column derivatization,8-diazabicyclo nonane
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