自创微需氧罐优选幽门螺杆菌培养与保存条件
World Chinese Journal of Digestology（2007）
Abstract
目的:简化幽门螺杆菌(H pylori)培养操作,优选H pylori培养与保存条件.方法:自创微需氧罐,采用正交设计优选Hpylori培养条件,并对-20℃保存的不同浓度两种保存液H pylori复苏对照观察.结果:自创微需氧罐成功培养出H pylori;哥伦比亚琼脂和脑心浸液琼脂中分别添加53 mL/L浓度小牛/绵羊血清和26 mL/L浓度混合抗生素,pH值7.5时,Hpylori生长良好;-20℃条件下:300 mL/L甘油/布氏肉汤和200 mL/L甘油/脑心浸液菌种保存时间分别为7d和20d.结论:自创微需氧罐操作简便、效果可靠,Hpylori培养与保存条件得到优化.
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Key words
Helicobacter pylori,Microaerophilic pot,Preservation,Cultivation
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