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A method to analyze, sort, and retain viability of obligate anaerobic microorganisms from complex microbial communities

Anne W. Thompson, Matthew J. Crow,Brian Wadey,Christina Arens,Serdar Turkarslan,Sergey Stolyar,Nicholas Elliott,Timothy W. Petersen,Ger van den Engh,David A. Stahl,Nitin S. Baliga

Journal of Microbiological Methods（2015）

Cited 10|Views34
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Abstract
A high speed flow cytometric cell sorter was modified to maintain a controlled anaerobic environment. This technology enabled coupling of the precise high-throughput analytical and cell separation capabilities of flow cytometry to the assessment of cell viability of evolved lineages of obligate anaerobic organisms from cocultures.
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Key words
Anaerobic microorganism,Flow cytometry,Controlled-environment sorting,Single cells
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