To establish an undergraduate proteomic experimental platform

Proteomic analysis greatly enriches our global view of the dynamic changes of cellular proteins during the embryo development or chemicals stimulation. Therefore, an integrated bioinformatics and proteomic experimental platform is an important core facility for the undergraduate biochemistry experiments. The E. coli and Zebrafish model was applied in the experimental platform. We performed 2D proteomic-based expression profiling on E. coli exposed to chemicals for 4 h to identify global proteins change and biological networks involved in chemicals-induced adaptive responses in vivo. The 2D electrophoresis gel data were analyzed in detail by PDQuest software and comparing to the proteomic database of other model system. We have established a multidimensional (expression, location, time course) secondary database. The secondary database will suggest some new relationships between genes and chemicals. The new development should serve as an experimental platform for undergraduate biochemistry experiments. Key word: Bioinformatics、Proteomics、Biochemistry experiments platform