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针对HBs基因的shRNA表达载体的构建及鉴定

Shang-jun YANG,En-jie TANG,Da-rong ZHANG

Journal of North Sichuan Medical College（2009）

Cited 1|Views47
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Abstract
目的 以PGenesil-1(Pg)为载体构建针对乙型肝炎病毒表面抗原(HBsAg)基因的shRNA表达载体PGenesil-1-HBs(Pgs).方法 设计、合成针对HBs区的三对DNA序列,分别插入PGenesil-1中,并对重组质粒进行限制性内切酶谱和DNA序列测定分析.结果 3个不同的重组shRNA表达载体经限制性酶识别和DNA序列测定完全正确.结论 成功构建了3个针对HBs基因的shRNA表达载体.
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Key words
HBsAg,Hepatitis B Virus,ShRNA,RNAi
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