Effect of metformin on secretion of human luteinized granulosa cells in vitro

Objective:To investigate the direct effect of metformin on ovarian steroidogenesis of granulose cells at different time. Methods:Human luteinized granulosa cells were isolated from patient during in vitro fertilization or intracytoplasmic sperm injection under Density gradient centrifugation and cultured in the absence or presence of metformin at different concentration.After treated with metformin at different concentration for every 24h,cell proliferation was studied by measuring the OD and culture medium was collected. Estradiol and Progesterone production was determined by chemi - luminescence immunoassay per 24h.Results:Steroid reached the peak during 72h to 96h and came down at 120h,which is in consistent with the growth curve.In vitro,metformin can inhibit the secretion of steroid.E2 and P was significantly inhibited at the concentration of 10000ng/ml.And there was a clear,dose - independent inhibition of E2 and P production by metformin.Conlusion:metformin inhibit the steroidogensis during the whole culture in vitro.