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一种快速制备酵母细胞PCR扩增DNA模板的方法

CHU Xue-ying,PANG Zhen-ling

Liquor-Making Science & Technology（2008）

Cited 23|Views11
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Abstract
报道了一种快速制备酵母细胞PCR扩增DNA模板的方法。以酿酒酵母(Saccharomyces cerevisiae)为目标菌,对酵母细胞煮沸-低温冷冻预处理后,直接用于PCR扩增,获得了26S rDNAD1/D2区域序列片段,成功测序。此方法避免了复杂的DNA提取过程。
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Key words
26SrDNA D1/D2 domain,DNA template,yeast,PCR amplification,mincrobe
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