一株高产低温纤维素酶南极细菌的筛选、鉴定及酶学性质初步研究

Chemistry & Bioengineering(2012)

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Abstract
从82株南极细菌中筛选出1株高产低温纤维素酶菌株NJ64,通过16S rDNA序列分析对其进行鉴定,并对其产酶条件及酶学性质进行了初步研究。结果表明,NJ64菌株为假交替单胞菌属(Pseudoalteromonas sp.);最适产酶条件为:培养时间72 h、初始pH值7.5、培养温度10℃;所产纤维素酶在pH值9.0左右、40℃条件下的酶活性最高,属于低温酶。
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Key words
antarctic,bacterium,cold-adapted cellulase
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