Simultaneous Quantification Of Complex Phospholipid Compositions Containing Monophosphoryl Lipid-A By Rp-Hplc

High performance liquid chromatography (HPLC) is a powerful technique for lipid quantification. However, for the characterization of lipid based vaccines more sensitive, high resolution methods for different lipid compositions are still evident. For this purpose, we have established a RP-HPLC method for the simultaneous quantification of saturated and unsaturated phospholipids with different acyl-chains and head groups, cholesterol and monophosphoryl Lipid-A without labeling. A novel ELSD feature enables adjustment of sensitivity within one run. By this method, we could demonstrate that complex lipid formulations can be quantified in concentrations of few micrograms but also in milligram amounts with the same performance characteristics.