A multiplatform real-time polymerase chain reaction detection assay for Vibrio cholerae
Diagnostic Microbiology and Infectious Disease(2009)
Abstract
We report a multiplatform real-time polymerase chain reaction methodology based on genes encoding for the regulatory toxR activator and enterotoxin A protein to determine enterotoxigenic Vibrio cholerae types from other vibrios. This assay, which was successfully validated on a collection of 87 bacterial strains, including 63 representatives of V. cholerae and 8 noncholera vibrios provides a rapid tool for detection and identification of cholera.
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Key words
Real-time PCR,Vibrio cholera,toxR,ctxA,Field detection
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