抵挡汤早期干预对2型糖尿病大鼠肿瘤坏死因子-α与血管细胞黏附分子-1表达的影响
Chinese Journal of Information on Traditional Chinese Medicine（2012）
摘要
目的观察抵挡汤早期干预对2型糖尿病大鼠血管病变中大血管的保护作用,探讨其机制。方法采用高脂饲料喂养及链脲佐菌素诱导方法制成大鼠糖尿病模型,抵挡汤早期干预组、抵挡汤中期干预组和抵挡汤晚期干预组分别于实验成模前4周、成模和成模后4周给予抵挡汤灌胃,辛伐他汀组和罗格列酮组大鼠于成模时分别给予辛伐他汀和罗格列酮灌胃,至实验24周时结束。酶联免疫吸附法检测大鼠血清血管细胞黏附分子-1(VCAM-1)水平,实时荧光定量PCR法检测主动脉肿瘤坏死因子-α(TNF-α)mRNA表达。结果与正常对照组比较,模型对照组大鼠血清VCAM-1水平明显升高(P<0.05),主动脉TNF-αmRNA表达明显升高(P<0.05);与模型对照组比较,抵挡汤早期干预组和辛伐他汀组大鼠血清VCAM-1水平明显降低(P<0.05),并且抵挡汤早期干预组、抵挡汤中期干预组和罗格列酮组大鼠主动脉TNF-αmRNA的表达明显降低(P<0.05)。结论抵挡汤早期干预能抑制大鼠主动脉TNF-αmRNA表达升高,抑制大血管病变的炎性损伤,延缓糖尿病大血管病变的发展,发挥其保护大血管的作用。
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关键词
reliable,rats HPLC WANG Xiao-lin1,ginseng saponins Rb1 in Longzhong Sunshang Capsule by HPLC with gradient elution.Methods C18 column(250 nm×4.6 nm,content determination,ginseng saponins Rb1 was in the range of 1.02-8.50 μg(r=0.999 5),and can be used for quality control of Longzhong Sunshang Capsule. Key words:Longzhong Sunshang Capsule,China) Abstract:Objective To establish the method for simultaneous determination of panax notoginseng saponins R1,Ginseng saponins Rb1,diabetes mellitus,accurate,GUO Min2,China,0.90-7.50 μg(r=0.999 0) and 1.17-9.75 μg(r=0.999 6) respectively.The average recovery was 100.98%(RSD=2.71%),TNF-α,macrovascular disease,Didang decoction,VCAM-1,panax notoginseng saponins R1,42-67 min(A 30%-46%).The detection wavelength was 203 nm,and the flow rate was 1.0 mL/min.Results The linear relationship of panax notoginseng saponins R1,97.73%(RSD=1.98%) and 99.57%(RSD=1.62%).Conclusion The method is simple,5 μm) was used with mobile phase of acetonitrile(A)-0.05% phosphoric acid solution(B),ZHANG Ping3,JIANG Hua2(1.Lanzhou University of Technology,ginseng saponins Rg1,2.Gansu Provincial Hospital of TCM,2,Ginseng saponins Rg1,LI Xiao-dong2,3.Gansu Institute for Food and Drug Control,Lanzhou 730050,gradient elution 0-32 min(A 23%-23%),32-42 min(A 23%-30%)
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