Affinity-purified Dermatophagoides farinae antigen induces CD23 on T and B lymphocytes and monocytes specifically in patients with atopic dermatitis

The purpose of this work is to analyze CD23 expression on subpopulations of peripheral blood mononuclear cells (PBMC) from the patients with atopic dermatitis (AD) after stimulation with affinity-purified Dermatophagoides farinae antigen (Af-DF). PBMC from mite-allergic AD patients stimulated with Af-DF induced striking expression of CD23 after the culture. The frequencies of CD23-positive (CD23+) PBMC in the culture were significantly higher than those without stimulation. Most of the CD23+ cells belonged to B lymphocytes and monocytes subsets. CD23 was induced in less than 1% of CD3+, CD4+ and CD8+ lymphocytes by Af-DF. However, significant differences were observed compared to the spontaneous expression, and dose-dependencies were confirmed. No significant increase in the frequencies of CD23+ PBMC was found in non-mite-allergic AD patients and non-atopic donors after stimulation with Af-DF. The kinetics of CD23 expression by Af-DF showed similar patterns to that by IL-4 stimulation. Our results demonstrate that Af-DF can induce CD23 expression on PBMC in mite-allergic AD patients. Based on the high titer of mite-specific IgE antibody in blood of AD, mite antigen may, at least in part, contribute to high CD23 expression in AD.