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彩叶草组织培养与快速繁殖技术研究

Modern Agricultural Science and Technology(2012)

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Abstract
以彩叶草节段为外植体进行离体培养,对外植体灭菌方法以及培养基中不同种类外源激素的浓度对试管苗增殖、生根的影响等进行了研究。结果表明:以0.1%HgCl2灭菌8 min,无菌外植体成活率可以达到70%;在MS+6-BA 2.0 mg/L+NAA 0.20 mg/L的增殖培养基上培养30 d,试管苗的增殖系数可达8.8;在1/2MS+6-BA 0.2 mg/L+NAA 0.5 mg/L的生根培养基上培养21 d后,生根率为100.0%,生根5.6条/株;炼苗后移植成活率达95%。
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Key words
Coleus blumei,node,tissue culture
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