The inhibitory effect of autophagy in liver induced by chronic arsenic exposure.

Objective:To investigate the effect of arsenic exposure on the autophagy in rat liver by establishing chronic arsenic-ex-posure model.Methods:The 21 days after birth in male SD rats were treated by different doses of arsenic which was carried out water containing NaAsO2.An automatic automatic biochemistry analyzer was used to determine the indexes for hepatic function;HE staining was used to determine the histopathological changes;transmission electron microscope was used to determine the ultrastructural changes;and Western blot was used to determine the expression of autophagy-related proteins including Beclin1 and LC3.Results:The exposure to arsenic resulted significant disfunction of liver,together with special histomorphology changes.Furthermore,the increased arsenic concentration resulted in the decrease of LC3Ⅱ/LC3Ⅰand Beclin 1 level.Conclusion:The exposure to arsenic may lead to the damage of liver and the inhibition of autophagy,which may play a role in arsenic-induced liver toxicity.