柴胡栽培种ZQ-T干根药材的AFLP鉴别
Journal of Shanxi University(Natural Science Edition)（2010）
Abstract
以干根药材为材料,采用AFLP技术进行了柴胡栽培种ZQ-T的鉴别.首先以3个柴胡栽培种干根样品混合DNA为模板进行AFLP分析,筛选出3对引物,经3次重复其DNA指纹图谱一致.然后以3对引物分别进行了7个栽培种的AFLP分析,每对引物均能从ZQ-T中扩增出特异条带,将ZQ-T鉴别出来;通过聚类分析,当遗传相似性系数分别为0.81、0.86、0.97时(引物分别为E-AAC/M-CTA、E-AGG/M-CTG和E-AGG/M-CAA)可将7个柴胡栽培种完全区分开.结果表明:AFLP可以较好地揭示柴胡种质资源的遗传差异,并可用于柴胡栽培种干根药材的鉴别.
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Key words
AFLP,medicinal materials identification,Bupleurum,Dried root
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