15-KETE对大鼠肺动脉平滑肌细胞Kv1.5、Kv2.1表达的影响
Laboratory Animal Science(2013)
Abstract
目的从细胞分子水平研究电压门控型钾通道亚型Kv1.5、Kv2.1在15-酮基二十碳四烯酸(15-ketoeicosatetretraenoic acid,15-KETE)致大鼠肺动脉收缩过程中的作用。方法通过酶法分离、培养SD大鼠肺动脉平滑肌细胞(pulmonary arterial smooth cells,PASMCs),使用RT-PCR和Western blot技术观察15-KETE对大鼠PASMCs上Kv1.5、Kv2.1通道表达的影响。结果 (1)15-KETE下调大鼠PASMCs膜上Kv1.5通道mRNA及蛋白质的表达;(2)15-KETE下调大鼠PASMCs膜上Kv2.1通道mRNA及蛋白质的表达。结论 Kv1.5、Kv2.1通道参与由15-KETE引起的缺氧肺动脉收缩(hypoxic pulmonary vasoconstriction,HPV)。
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Key words
hypoxic pulmonary vasoconstriction,pulmonary arterial smooth cells,15-ketoeicosatetretraenoic acid,voltage-gated potassium channel subtype
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