Effect of arsenic trioxide on expressions of matrix metalloproteinase 2 and 9 in HL-60 cells

Objective:To investigate the effect of arsenic trioxide(As2O3)on expressions of matrix metalloproteinase 2 and 9(MMP-2 and MMP-9) in HL-60 cells.Methods:HL-60 cells were treated with different concentrations of As2O3 for 48 hours.Semiquantitative reverse transcription polymerase chain reaction(RT-PCR) was used to examine the expressions of MMP-2 and MMP-9 mRNA.Western blot was used to evaluate the productions of MMP-2 and MMP-9 proteins.Results:The expressions of MMP-2 and MMP-9 mRNA in HL-60 cells were decreased after treated with As2O3.There were statistical significances between 5.0 and 7.5 μmol/L As2O3 groups and control group(P0.05-P0.01).The productions of MMP-2 and MMP-9 proteins were reduced dose-dependently when treated with As2O3.There was statistical significance between each experimental group and control group(P0.05-P0.01).Conclusions:As2O3 can inhibit the expressions of MMP-2 and MMP-9 in HL-60 cells.This effect might be one of the molecule mechanisms of As2O3 in the treatment of leukemia.