Simultaneous Detection of Hepatitis B Virus,Hepatitis C Virus,Human Immunodeficiency Virus Type-1,Hepatitis A Virus,Hepatitis G Virus and Human Parvovirus B19 by Oligonucleotide Microarray Hybridization

We described the diagnosis microarray for simultaneous detection of hepatitis B virus,hepatitis C virus,human immunodeficiency virus type-1,hepatitis A virus,hepatitis G virus and human parvovirus B19.Fully sequenced viral genomes were obtained from GenBank and aligned to find out the most conserved regions on which designed oligonucleotide probes were based.With all the specific probes corresponding to the six types of viruses to be detected in our experiment,diagnosis microarrays were fabricated,in which negative and positive probes were used as control.On the other hand,the tested viral nucleotides were amplified,fluorescently labeled and hybridized with the diagnosis microarray.Then the microarrays were scanned using a GenePix 4100A scanner,and the data obtained were analyzed.The diagnosis microarray showed high degree of specificity and identified successfully the viruses in clinical samples,and could detect efficiently 10~2 copies of the plasmid and 10~3 virus copies in clinical samples.Clinical samples coinfected with several viruses could be detected simultaneously.The assay demonstrated the potential of microarray for diagnosis of HBV,HCV,HIV,HAV,GBV-C/HGV and B19.