Medium optimization of Neisseria meningitidis serogroup A by quadratic orthogonal rotation combination design.

Aim to optimize medium of Neisseria meningitidis serogroup A by quadratic orthogonal rotation combination design.Methods Using shake flasks for fermentation after design four factors quadratic orthogonal rotation combination to optimize culture formula,then the data was analysed by SAS V9.1 software.At last,3 tests verified for optimized formula.Results The optimum concentrations of 4 factors were L-glutamic acid 5g/L,casein 15g/L,yeast extract 15g/L,glucose 5g/L.3 tests using optimized formula were verified,and the average value of A600 was 2.278,without apparent difference compared with the predicted value 2.299.Conclusion The quadratic orthogonal rotation combination design can be used to optimize the medium for Neisseria meningitidis serogroup A.