Effect of Genistein on the Osteoprotegerin /Receptor-activator of Nuclear Factor Kappa Bata/Receptor-activator of Nuclear Factor Kappa Bata Ligand System of Fibroblast-like Synoviocytes

Objective In order to investigate the effect of genistein (gen) on the secretion of receptor-activator of nuclear factor kappa bata ligand (RANKL)/osteoprotegerin (OPG) secreted by fibroblast-like synoviocytes. Methods The expression of CD29,CD90 of human rheumatoid arthritis FLS was detected by Flow cytometry. The experience was divided into four groups: the control group,the TNF α treatment group,the TNF α +Gen group and the Gen group. Western blot was used to detect the expression of estrogen receptor α (ER α),receptor-activator of nuclear factor kappa bata (RANK),RANKL,OPG. Immunofluorescence was used to detect ER α. Results Western Blotting showed that Gen could significantly up-regulate the expression of OPG and RANKL relative to the control group (P0. 05); but had no effect on RANK and ER α. The statistical analysis showed that the ratio of OPG/RANKL in the Gen group was increased. Compared with the control group,the results from the immunofluorescence also showed that most of the ER α were transferred into the nucleus. Conclusion Gen may influence ERα to regulate the OPG/RANKL/RANK pathway.