Supplemental Data Regulation of XIAP Translation and Induction by MDM2 following Irradiation

Figure S1. Effect of IR-induced DNA damage on XIAP expression and sensitivity to apoptosis in cells with distinct MDM2 expression. (A) Western blot assay for the protein expression of MDM2, XIAP, and p53 in six cancer cells lines (2 ALL and 4 NB) used as models in this study. (B) Kinetic analyses of XIAP protein expression in SH-EP1 and SH-SY5Y cells following 10 Gy IR treatment, as detected by western blot assay. (C) Results of western blot assay for activation of caspase-3, -9 and cleavage of death substrate PARP in SH-EP1 and SH-SY5Y cells that were treated with 10 Gy IR for the indicated time. (D) Dose-dependent apoptosis induced by IR in SH-EP1 and SH-SY5Y NB cell lines. Cells were exposed to different dose of IR for 24 h, and apoptotic cells were determined by annexin-V staining and analyzed by flow cytometry. Data represents the mean (±SD) percentage of annexin-V positive cells from three independent experiments. * p<0.01.