Voltage-Gated Potassium Channel In Brain Mitochondria
Biophysical Journal(2009)
摘要
Transient cerebral ischemia is known to induce endogenous adaptive mechanisms such as the activation of mitochondrial ATP regulated potassium channels or Ca2+ regulated large conductance potassium channels that can prevent or delay neuronal injury. In this study a single channel activity was measured after patch-clamp of the mitoplasts isolated from gerbil hippocampus. In 70% of the all patches, a potassium selective current was recorded with mean conductance 109 ± 6 pS in symmetrical 150 mM KCl solution. The patch-clamp single channel studies showed properties of the voltage-gated potassium channel (Kv channel). We found that ATP/Mg2+ complex and Ca2+ ions had no effects on observed activity of ion channel. Observed channel was blocked by negative voltage and margatoxin (MgTx) a specific Kv1.3 channel inhibitor. The inhibition by MgTx was irreversible. We conclude that gerbil hippocampus mitochondria contain voltage-gated potassium channel (mitoKv) with properties similar to the surface membrane Kv1.3 channel which can play a role in control function of mitochondria as well as in ischemia-reperfusion phenomenon. This study was supported by grant No. P-N/031/2006 and Polish Mitochondrial Network MitoNet.pl.
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关键词
potassium channel,brain mitochondria,voltage-gated
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