Formation Of Ginsenoside-Protein Conjugates
ADVANCES IN PLANT GLYCOSIDES, CHEMISTRY AND BIOLOGY(1999)
Abstract
Ginsenoside Rbl was conjugated with bovine serum albumin by NaIO4. MALDI/MS spectrum of the conjugates revealed that most abundant conjugate had 10.2 moles of hapten(ginsenoside) and hapten number ranged from 0.6 moles to 24.6 moles. Ginsenoside-protein conjugate formation was also proved using ginsenoside Rc-HSA conjugate by PAGE gelelectrophoresis. With mercaptoethanol hapten number of conjugate seemed increased but solubility decreased. Monoclonal antibody formation is under investigation with ginsenoside-BSA conjugate.
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Key words
ginsenoside-protein conjugate,MALDI/MS,gel electrophoresis
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