Preparation and Characterization of the Monoclonal Antibodies to PTD-bcr/abl

AIM:To Preparation and characterization the monoclonal antibody (mAb)to PTD-bcr/abl fusion protein.METHODS:The PTD-bcr/abl fusion protein was expressed in E.coli. BALB/c mice were immunized with specific PTD-bcr/abl. The splenic cells of the mice were fused with Sp2/0 mouse myeloma cells. The hybridoma culture supernatants were screened by indirect ELISA. The property of the antibody was identified by Western blot and immunohistochemistry.RESULTS:The PTD-bcr/abl fusion protein and its mAb were obtained. mAb against PTD-bcr/abl displayed strong specifility and high affinity.CONCLUSION:An anti-PTD-bcr/abl fusion protein mAb with high affinity was abtained, which could be applied to the research of PTD-bcr/abl.