Solid-Phase Microextraction Followed by High-Performance Liquid Chromatography with Fluorimetric and UV Detection for the Determination of Polycyclic Aromatic Hydrocarbons in Water

We have developed a solid-phase microextraction procedure for polycyclic aromatic hydrocarbons. A simplex experimental design was employed to optimize the process. A polydimethylsiloxane/divinylbenzene fiber was selected. The optimum conditions were: an extraction step in the immersion mode, over a period of 60min at 70 degrees C using high-speed stirring, and an 8min desorption step using acetonitrile (90 mu l). Linear relationships were obtained for all compounds, except for naphthalene. Our method showed a good precision and accuracy with a detection limit between 0.005 and 0.306 mu g1(-1). Our method was used to detect PAHs in real water samples.