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HPLC测定鱼腥草配方颗粒中槲皮素含量

Chinese Journal of Experimental Traditional Medical Formulae(2012)

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Abstract
目的:建立测定鱼腥草配方颗粒中槲皮素含量的HPLC方法。方法:采用正交试验优化鱼腥草配方颗粒的提取工艺,用HPLC测定鱼腥草配方颗粒中槲皮素的含量。色谱柱为Hypersil ODS C18柱(4.6 mm×250 mm,5μm),流动相为甲醇-0.2%磷酸(45∶55),检测波长372 nm,流速1.0 mL.min-1,柱温30℃。结果:槲皮素在0.09~1.44μg线性关系良好(r=0.999 9),平均加样回收率为100.6%,RSD 1.68%(n=6)。结论:该方法简便、快速、重复性好,可作为鱼腥草配方颗粒中槲皮素的含量测定方法。
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Key words
HPLC,quercetin,determination,Yuxingcao dispensing granules
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