A pilot study of red blood cells storage at high temperature

Objective:To study the storage method of red blood cell(RBC) at high temperature and provide the scientific evidences for blood preservation and transportation in battle fields.Methods:200 ml of whole blood was obtained from each of the 10 donors and the RBC suspension was prepared.The RBC suspension was divided into 3 groups: A group stored at room temperature,B group(stored at room temperature initially for 3 days and at 4℃ later) and C group(stored at 4℃).Several parameters such as ATP were tested daily.The RBC ATP(concentration) at the end of shelf life in C group served as critical ATP.When RBC ATP decreased to the level of critical ATP,the preservation time was recorded as its shelf life.Results:The RBC suspension could be stored for 4 days at high temperature ranged from 26.2℃ to 36.3℃,and it could continue being stored at 4℃ for 7 days after 3 days of storage at room temperature.Conclusion:The RBC suspension can be stored and transported in its shelf life at high temperature and the wounded can obtain blood transfusion in time.