Protective effect of exogenous hydrogen sulfide on oxidative stress injury induced by high glucose in human peritonea 1 mesothelial cells line HMrSV5 cells

Objective To investigate the protective effect of exogenous hydrogen sulfide (H2S) on oxidative stress injury induced by high glucose in human peritoneal mesothelial cells line HMrSV5 cells. Methods HMrSV5 cells were incubated in 4.25% D-glucose (high glucose) to induce injury, and the cells were treated with the H2S donor sodium bisulfide (NaHS) for 24 h. MTT assay was used to assay the cell viability. The level of reactive oxygen species (ROS) was measured by flow cytometry. Lactate dehydrogenase (LDH) in the medium, malondialdehyde (MDA) in the cells, and superoxide dismutase (SOD) activity in the cells were also measured. Results In high glucose group, cell viability and SOD activity in cells were significantly decreased, and LDH in medium, ROS in cells, and MDA in cells were significantly increased, as compared with those in the control group. NaHS reversed the effects of high glucose on HMrSV5 cells. Conclusions Exogenous H2S prevents oxidative stress injury induced by high glucose in human peritoneal mesothelial cells, probably through the inhibition of oxidative stress by exogenous H2S.