Hepatoma-associated non-histone proteins are phosphoproteins preferentially localized in nuclear matrix.

1.1. High molecular weight non-histone proteins (NHP) were isolated from Morris hepatoma 7777 by Sephadex G-100, S-200 chromatography.2.2. Specific polyclonal antibodies were raised against these NHP in rabbits. These antibodies recognized specific NHP components present in Morris hepatoma 7777 and 8994, but not in normal rat liver. Hepatoma-associated antigens are phosphoproteins.3.3. Immunologically specific NHP of Morris hepatoma are intensively concentrated in nuclear matrix fraction.