The MEV Mouse Linkage Testing Stock: Mapping 30 Novel Proviral Insertions and Establishment of an Improved Stock

Continuing efforts to improve the MEV linkage testing stock are described. The MEV stock carries multiple copies of the ecotropic murine leukemia virus genome. These proviruses are individually detectable in Southern blots, making it possible to follow the segregation of many loci simultaneously in genetic crosses. About 50 novel proviral insertions have been detected in this stock and propagated to homozygosity in a number of sublines. Many of the proviruses were first observed in individuals that were somatic and germinal mosaics. From the minimum frequency of primary transmission of proviruses to offspring, it is estimated that about 7 embryonic cells contribute to the pool of germ cells that populate the gonads. Thirty of the proviruses have been mapped to 15 different chromosomes in intercrosses between MEV sublines and the inbred Mus musculus castaneus strain CAST/Ei or in other crosses. Mapped insertions appear to be randomly distributed among chromosomes, but possibly clustered within chromosomes. Several of the mapped insertions are located such that their inclusion in improved MEV stocks would permit the screening of additional regions of the genome. An improved MEV stock that is homozygous for 12 proviruses on 11 chromosomes, including novel insertions on Chromosomes 17 and 19 is described. The stock also carries four dominant visible markers on 4 additional chromosomes. This stock is estimated to sweep approximately 68% of the autosomal genome.