人牙周膜干细胞的多向分化潜能实验
Journal of Clinical Stomatology(2012)
Abstract
目的:体外培养分离人牙周膜干细胞(hPDLSC),做成骨诱导和成脂诱导实验。方法:取新鲜拔除正畸牙的牙周膜为材料制成组织块,加入培养液以组织块法分离培养,免疫磁珠法筛选人牙周膜干细胞,加入成骨诱导液诱导牙周膜干细胞成骨,加入成脂诱导液诱导牙周膜干细胞向脂肪细胞转化。结果:成功筛选出人牙周膜干细胞,成骨及成脂诱导成功。结论:免疫磁珠法是筛选人牙周膜干细胞的有效方法,牙周膜干细胞可以向成骨细胞和脂肪细胞分化。
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Key words
osteogenic induction,adipogenic induction,immunomagnetic beads,human periodontal ligament stem cells(hPDLSC)
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